Figure 3.

Effect of BRAF and MEK inhibitors on MEK and ERK phoshphorylation in Calu-6 cells. (A) Comparison of Vemurafenib (Vemu) and EBI-907 on MEK and ERK phosphorylation. (B) Effect of EBI-907 on ERK phosphorylation with or without MEK inhibitor EBI-1051. The cells were treated with increasing concentrations of the indicated BRAF inhibitors and/or MEK inhibitor for 2 hrs and the levels of p-MEK and p-ERK in cell lysates were measured by Western blot analysis. Total MEK protein level was also monitored and served as loading control.