Figure 5. Other ribosomal protein mutants do not share translational phenotypes with the ASC1 mutants.
(A) Correlations between ∆TE among asc1-M1X and mutants with reduced expression of large ribosomal subunit proteins, rpl23b∆ and rpp1a∆. The Pearson correlation coefficient is shown. (B) Correlations between ∆TE among asc1-M1X and mutants with reduced expression of small ribosomal proteins in the vicinity of Asc1, rps0b∆ and rps16b∆. The Pearson correlation coefficient is shown. (C) Violin plots showing the change in TE for the ‘strong closed loop’ and ‘closed loop’ mRNAs in asc1-M1X and the other ribosomal protein mutants. Violin plot parameters are described in Figure 4B.
Figure 5—figure supplement 1. Phenotypes of selected large ribosomal protein mutants.
(A) Growth curve comparing growth of asc1-M1X with other RP mutants rpl23b∆ and rpp1a∆ at 30˚C in glucose. (B) Polysome profiles of the rpl23a∆ and rpp1a∆ mutants at 30˚C. The polysome/monosome (P/M) and 60S/40S (60/40) ratios are shown with s.d. from two biological replicates.
Figure 5—figure supplement 2. Ribosomal location and phenotypes of selected small ribosomal protein mutants.
(A) Structure of the yeast 40S ribosome with the positions of Rps0 and Rps16 shown relative to Asc1. Structure taken from Ben-Shem et al. (2011). (B) Polysome profiles from WT, rps0b∆, and rps16b∆.