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. Author manuscript; available in PMC: 2016 Oct 11.
Published in final edited form as: Nat Genet. 2016 Apr 11;48(5):544–551. doi: 10.1038/ng.3548

Figure 4.

Figure 4

A single gene knockout of ald confers a growth advantage relative to wild type M. tuberculosis when cultured in the presence of D-cycloserine. Four laboratory strains—wild type M. tuberculosis (WT), ald knockout (Δald ), ald complemented back into Δald (ald-comp) and BCG—were cultured in triplicate in 0, 7.5 and 15 μg/mL of D-cycloserine. The resulting time to positivity (ttp) in MGIT was recorded as days since inoculation, and the resulting ttp was normalized to the no drug control for each strain to calculate the growth inhibition index for each drug concentration. The mean growth inhibition index is plotted with error bars to represent the SEM. P-values were calculated using a two-way ANOVA.