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. Author manuscript; available in PMC: 2016 Oct 18.
Published in final edited form as: Nat Cell Biol. 2016 Apr 18;18(5):572–578. doi: 10.1038/ncb3341

Figure 4. GSH biosynthesis is required for the PI3K/Akt-driven initiation of tumor spheroids.

Figure 4

a, Cells were grown as spheroids in 3D culture for 14 days in the presence or absence of 50 μM BSO. Representative images from two biologically independent experiments are shown. b, Cells were grown as colonies in soft agar in the presence or absence of 50 μM BSO for 4 weeks. Viable colonies were stained with iodonitrotetrazolium chloride. Representative images from two biologically independent experiments are shown (left). The number of colonies was counted with MatLab (right, data are from one experiment that was independently repeated two times with similar results (Supplementary Table 1)).