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. 2016 Apr 28;7:520. doi: 10.3389/fpls.2016.00520

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Copyright © 2016 Wuddineh, Mazarei, Zhang, Turner, Sykes, Decker, Davis, Udvardi and Stewart.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Relative transcript levels of the PvKN1a in transgenic and non-transgenic (WT) switchgrass (ST1) plants. The expression analysis was done using RNA derived from the shoot tips at E4 developmental stage. The dissociation curve for the qRT-PCR products showed that the primers were gene-specific. The relative levels of transcripts were normalized to UBQ. Bars represent mean values of three replicates ± standard error. Bars represented by different letters are significantly different at P ≤ 0.05 as tested by LSD method with SAS software (SAS Institute Inc.).