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. 2016 Apr 28;7:533. doi: 10.3389/fpls.2016.00533

FIGURE 1.

FIGURE 1

Defective seedling growth in nyc1 and its recovery in VIPP1-GFP/nyc1. (A) Germination and seedling development of Col, nyc1, VIPP1-GFP/nyc1, nol, and VIPP1-GFP/nol on MS-agar plate supplemented with 1.5% sucrose under normal light with 12 h/12 h light/dark cycle at 22°C. Photographs of the seedlings were taken 4, 8, or 12 days after sowing, respectively, as shown at the left. Bars = 5 mm. (B) Close-up view of representative seedlings of Col, nyc1, and VIPP1-GFP/nyc1 during seedling development at 4, 8, and 12 days after sowing. In nyc1, the cotyledon was defective and white (red arrows), whereas the first true leaf was green (white arrows). Bars = 2 mm. (C) Immunoblots of total protein extract from Col, nyc1, and VIPP1-GFP/nyc1 (12-days-old individuals as shown in (B) were probed with anti-VIPP1. Loading was based on the equal total protein. Proteins stained with Coomassie Brilliant Blue (CBB) after SDS-PAGE are shown as loading control at the bottom. M denotes the molecular marker shown along with size.