FIGURE 4.

Stay-green phenotype and retention of Chl and fluorescence in nyc1 and VIPP1-GFP/nyc1. (A) Dark-induced senescence of mature leaves from Col, nyc1, and VIPP1-GFP/nyc1. Detached leaves of 3-weeks-old plants were incubated in darkness for 5 days at 22°C. Photographs of representative leaves before and after dark treatment are shown, respectively, as control (top panel) and darkness (bottom panel). (B) Total Chl content (left) and Chl a/b ratio (right) were calculated from the leaves as shown in (A). Three independent experiments were performed and error bars (SD) are represented as SD. Values before and after dark treatment are shown, respectively, as control (blue) and darkness (red). (C) Fluorescence emission of seeds from Col, nyc1, and VIPP1-GFP/nyc1 detected by microscopy (bottom panel). Photographs of the same seeds taken by bright field are shown at the top.