Figure 3.

Prime-boost vaccine regimens induce comparable immune responses in both BALB/c and B6C3F1 mouse strains. MOMP-specific IgG concentrations were measured in serum (A) and vaginal washes (B) for both BALB/c and B6C3F1 vaccinated mice (n = 8 per group), expressed as individual concentrations with bars representing the means. (C) Serum MOMP-specific IgG2a and IgG1 isotype concentrations were measured by ELISA for both BALB/c and B6C3F1 vaccinated mice (n = 5–8 per group), and individual points and bars representing the means IgG2a:IgG1 ratios plotted. The dotted line indicates the IgG2a:IgG1 ratio of 1, demonstrating Th1-skewing above this line or Th2-skewing below it. (D) Splenocytes were assessed by IFN-γ ELISpot for MOMP-reactive T cells stimulated by a peptide pool consisting of 15-mers overlapping by 11 amino acids for both vaccinated BALB/c and B6C3F1 mice. Data expressed as individual values with bars representing group medians (+ interquartile range) (SFU/million antigen-stimulated cells).