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Verification of lymphatics in the Matrigel plugs with CD31 (green) and Prox1 (red) antibodies. The pan-endothelial marker CD31 stains blood vessels (BV) strongly and lymphatics (LV) weakly. Prox1 marks the nuclei of LECs (arrowheads) as shown at higher magnification in the inset. Nuclei are counter-stained with Dapi (blue). Bar 30 µm
