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. 2016 Apr 14;2016:5201879. doi: 10.1155/2016/5201879

Table 2.

Average content of polyphenols and respective retention time (Rt) and wavelengths of maximum absorption in the visible region (λ max) in P. tridentatum extracts (by elution order).

Polyphenols Rt (min) UV (nm) UV-DAD/VIS bands (nm)
in 70% methanol
Class Mg·g−1 dry weight
Taxifolin 17.48 280 290, 327sh Flavanol 21.76 ± 0.030
Genistin 19.79 320 259, 323sh Isoflavone 16.75 ± 0.040
Genistein 22.73 320 259, 332sh Isoflavone 12.01 ± 0.030
Quercetin-3-O-rutinoside 23.88 370 256, 266sh, 351 Flavonol 1.58 ± 0.030
Quercetin-3-O-glucoside 23.99 370 255, 266sh, 348 Flavonol 1.23 ± 0.010
Ferulic acid 24.32 320 244, 296sh, 324 Hydroxycinnamic acid 0.27 ± 0.002
Biochanin A-glucoside 30.66 320 265, 322sh Flavone 1.37 ± 0.002
Apigenin 32.65 370 229, 267sh, 341 Flavone 0.44 ± 0.002
Biochanin A 34.88 320 265, 323sh Flavone 2.89 ± 0.004

Total of hydroxycinnamic acids 0.27 ± 0.002
Total of flavonols 24.57 ± 0.100
Total of flavone 4.70 ± 0.008
Total of isoflavone 28.76 ± 0.070
Total of polyphenols identified 58.30 ± 0.180

Values expressed as mean ± standard deviation of three replicates.