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. 2016 Apr 14;2016:5201879. doi: 10.1155/2016/5201879

Table 3.

Average content of polyphenols and respective retention time (Rt) and wavelengths of maximum absorption in the visible region (λ max) in M. pulegium extracts (by elution order).

Polyphenols Rt (min) UV (nm) UV-DAD/VIS bands (nm)
in 70% methanol
Class Mg·g−1 dry weight
p-Hydroxybenzoic acid 13.46 280 227, 260sh, 295 Hydroxybenzoic acid 0.212 ± 0.004
Chlorogenic acid 17.95 320 242, 300sh, 327 Hydroxycinnamic acid 0.387 ± 0.004
(+)-Catechin 18.10 280 231, 280 Flavan-3-ols 0.212 ± 0.004
Caffeic acid 21.32 320 243, 296sh, 324 Hydroxycinnamic acid 0.230 ± 0.001
Quercetin-3-O-rutinoside 22.96 370 256, 266sh, 351 Flavonol 0.144 ± 0.003
Quercetin-3-O-glucoside 23.63 370 255, 266sh, 348 Flavonol 0.140 ± 0.002
Neohesperidin 24.84 280 283, 326 Flavanone 0.628 ± 0.001
Luteolin-7-O-glucoside 25.31 370 254, 266, 349 Flavone 0.201 ± 0.004
Diosmin 26.04 370 253, 268, 339 Flavone 0.623 ± 0.006
Rosmarinic acid 26.78 320 249, 299sh, 330 Hydroxycinnamic acid 0.287 ± 0.006
Ferulic acid 27.55 320 241, 296sh, 324 Hydroxycinnamic acid 0.277 ± 0.003
Apigenin 32.65 370 235, 267sh, 341 Flavone 0.323 ± 0.002

Total of hydroxybenzoic acids 0.212 ± 0.004
Total of hydroxycinnamic acids 1.181 ± 0.014
Total of flavan-3-ols 0.212 ± 0.004
Total of flavonols 0.284 ± 0.005
Total of flavones 1.147 ± 0.012
Total of flavanones 0.628 ± 0.001
Total polyphenols identified 3.664 ± 0.040

Values expressed as mean ± standard deviation of three replicates.