Table 1. Quality and composition of Lepidoptera genomes.

Feature	Pgl	Ppo	Pxu	Dpl	Hme	Mci	Lac	Bmo	Mse	Pxy	Cce	Pse
Size w/o gap (Mb)	361	218	238	242	270	361	290	432	400	387	689	347
Scaffold N50 (kb)	231	3672	6199	716	194	119	525	3999	664	734	233	257
GC content (%)	35.4	34.0	33.8	31.6	32.8	32.6	34.4	37.7	35.3	38.3	37.1	39.0
Repeat (%)	22.2	n.a.	n.a.	16.3	24.9	28.0	15.5	44.1	24.9	34.0	34.0	17.2
Exon (%)	5.11	7.79	8.59	8.41	6.19	4.34	7.24	4.07	5.34	6.47	3.11	6.20
Intron (%)	24.8	51.6	45.5	26.6	24.1	31.2	32.3	16.1	38.3	31.3	24.0	25.5
Genome size (Mb)	375	227	244	249	274	390	298	481	419	394	729	406
Heterozygosity (%)	2.3	n.a.	n.a.	0.55	n.a.	n.a.	1.5	n.a.	n.a.	~2	1.2	1.2
CEGMA (%)	99.6	99.3	99.6	99.6	98.2	98.9	99.6	99.6	99.8	98.7	100	99.3
CEGMA coverage by single scaffold (%)	86.9	85.8	88.8	87.4	86.5	79.2	86.8	86.8	86.4	84.1	85.3	87.4
Ribosomal Proteins (%)	98.9	98.9	97.8	98.9	94.6	94.6	98.9	98.9	98.9	93.5	98.9	98.9
De novo transcripts (%)	98	n.a.	n.a.	96	n.a.	97	98	98	n.a.	83	96	97
number of proteins (k)	15.7	12.3	13.1	15.1	12.8	16.7	17.4	14.3	15.6	18.1	16.5	16.5

Pgl: Pterourus glaucus; Ppo: Papilio polytes; Pxu: Papilio xuthus; Dpl: Danaus plexippus; Hme: Heliconius melpomene; Mci: Melitaea cinxia; Lac: Lerema accius; Bmo: Bombyx mori; Mse: Manduca sexta; Pxy: Plutella xylostella; Cce: Calycopis cecrops; Pse: Phoebis sennae.

Heterozygosity: Calculated as the percent of heterozygous positions detected by the Genome Analysis Toolkit (GATK) for Pgl, Lac, Cce and Pse; or taken from information in the literature for Dpl⁶; or estimated based on the histogram of K-mer frequencies for Pxy¹⁶,⁴⁷.

n.a.: data not available.