Figure 5. Properties of I_h in EGFP-expressing IN in dLGN.

(A) Frequency distribution of membrane capacitance of EGFP-expressing IN recorded under voltage clamp conditions. The ordinate represents the number of cells within each 20 pF class along the abscissa. Note the bimodal membrane capacitance distribution for the total IN population in dLGN. (B) Representative I_h recordings from IN in dLGN from small (red traces) and large (blue traces) IN are shown. (C) Mean I_h current density from small and large IN determined at −130 mV (Mann-Whitney test, two tailed: P < 0.001). (D) Mean steady-state activation curves from small (red data points) and large (blue data points) IN are shown. Solid lines represent best approximations of Boltzmann functions to the data points. (E,F) Box plots of the potentials of half-maximal activation (E) Mann-Whitney test, two tailed: P = 0.38) and time constants of activation (F; Mann-Whitney test, two tailed: P = 0.98) revealed no significant differences between small (red symbols) and large (blue symbols) IN.