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. 2016 Apr 28;6:24900. doi: 10.1038/srep24900

Figure 4. Hypoxia upregulates RhoB/Rho-kinase signaling in PASMC.

Figure 4

(A) Representative western blot for hypoxia-induced increase of RhoB in mouse lungs, rat PASMC and human PASMC (n = 3). β-actin was used as a loading control. (B) RhoB mRNA levels in hypoxia-exposed rat and human PASMC were evaluated by qRT-PCR using β-actin as an internal control. Bar charts showing the relative expression level by normalizing to the normoxia control (N-Con). Data are shown as means ± SE (n = 3). (C) Expression of RhoB/Rho-kinase signaling pathway related components in rat and human PASMC in response to hypoxia was determined by western blotting (n = 3). *p < 0.05, **p < 0.01.