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. 2016 Apr 28;6:24844. doi: 10.1038/srep24844

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Copyright © 2016, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(A) Representative images from Co-IP analysis of HCN channels and Cav-3. Rat bladder lysate was Co-IPed with anti-Cav-3 antibody or rat IgG, and then the Co-IP samples were analyzed by Western blotting with anti-HCN antibodies (left lane: bladder lysate; mid lane: rabbit Ig G; right lane: Cav-3 Co-IP samples). (B,C) Confocal images from rat bladder and primary isolated ICCs-DM reveals the co-localization of HCN4 and Cav-3 by immunostaining with rabbit anti-HCN4 polyclonal antibody and rabbit anti-Cav3 polyclonal antibody.