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. 2016 Apr 28;6:24844. doi: 10.1038/srep24844

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Copyright © 2016, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Transfection with Cav-3 siRNA in ICCs-DM decreased the protein and function levels of HCN channels. (A,B) The decreased expression level of Cav-3 was observed only in Cav-3 siRNA transfecting ICCs-DM. (C,D) Transfection with Cav-3 siRNA can significantly inhibit the protein expression level of HCN1 and HCN4. (E,F) Representative current traces evoked by hyperpolarizing voltage steps from −120 to −60 mV were recorded in primary isolated normal and Cav-3 siRNA transfecting ICCs-DM. (G,H) Administration with ZD7288 inhibited the Ih currents in both normal and Cav-3 siRNA transfecting ICCs-DM. Transfection with Cav-3 siRNA decreased the V_1/2 and Ih currents at the potential of −110, −100 and −90 mV. (J) The Ih currents at −110, −100 mV were also decreased by adding ZD7255 (n = 4, independent-sample t-test, **P < 0.01 and ***P < 0.001).