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. 2016 Apr 25;7:11386. doi: 10.1038/ncomms11386

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(a,b) Subcellular localization of Flag-DNAJC14 and Myc-Hsc70 was examined in permeabilized PANC-1 cells. Representative images are shown in a. Quantitation of results from multiple experiments is summarized in b. A high degree of colocalization (an average of 52%) was observed in cells stained doubly with Hsc70 and DNAJC14. The Hsc70-K71M and DNAJC14-H471Q mutations significantly reduced colocalization of Hsc70 and DNAJC14. Scale bar, 20 μm. **P<0.01 by one-way analysis of variance, difference from lane 1, n=15. (c–e) Endogenous DNAJC14 and Rab18 in PANC-1 cells were immunostained. ER-to-Golgi blockade by Arf1-Q71L (c) or Sar1-T39N (d) induced the movement of endogenous DNAJC14 into punctate structures and increased colocalization with Rab18 (d,e). Scale bar, 20 μm. **P<0.01 by paired Student's t-test, n=15.