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. 2016 Apr 28;6:25224. doi: 10.1038/srep25224

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Copyright © 2016, Macmillan Publishers Limited

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(a) DMSO and E27 (1 μM) were added to Huh7 cells and allowed to incubate for 24 h. CLDN1, OCLND, CD81 and SR-BI were stained with corresponding antibodies (green), and nuclei (blue) were stained with DAPI. (b) Huh7 cells were treated with 0, 0.1, 1 or 5 μM of E27, and the expression levels of CLDN1, OCLND, CD81 and SR-BI were analyzed by Western blotting. The expression of β-actin was also analyzed as a loading control.