Table 1. DNA sequence designs for the fabrication of siRNA-NS.
DNA strands | Sequences | Length (nt) |
---|---|---|
Linear DNA for anti-GFP siRNA sense strand | 5′- Phosphate - ATA GTG AGT CGT ATT AAA AAC TTC AGG GTC AGC TTG CTT GCT GGA TGA AGG ACG GTC GAA CGC AAA ACT TCA GGG TCA GCT TGC TTA TCC CT- 3′ | 92 |
Linear DNA for anti-GFP siRNA antisense strand | 5′- Phosphate -ATA GTG AGT CGT ATT AAA GCA AGC TGA CCC TGA AGT TTT CTT AGG CTG GAC AAC AAC CAT CTA AAG CAA GCT GAC CCT GAA GTT TTA TCC CT- 3′ | 92 |
Linear DNA for scrambled siRNA sense strand | 5′- Phosphate - ATA GTG AGT CGT ATT AAA ATG TGA ATG CAG ACC AAA GAA TTG CTG GAT GAA GGA CGG TCG AAA ATG TGA ATG CAG ACC AAA GAA TTA TCC CT -3′ | 92 |
Linear DNA for scrambled siRNA antisense strand | 5′- Phosphate -ATA GTG AGT CGT ATT AAA TTC TTT GGT CTG CAT TCA CAT TTG GCT GGA CAA CAA CCA TCT AAA TTC TTT GGT CTG CAT TCA CAT TTA TCC CT-3′ | 92 |
Primer DNA | 5′-TAA TAC GAC TCA CTA TAG GGA T-3′ | 22 |
DNA sequences contain the region complementary to primer DNA to form T7 promoter region, sense and anti-sense regions to form siRNAs.