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. 2016 Apr 25;7:11388. doi: 10.1038/ncomms11388

Figure 3. ptre1 mutants have auxin-related phenotypes.

Figure 3

(a) ptre1 shows reduced hypocotyl elongation under high temperature (28 °C). Seedlings were grown at 22 or 28 °C for 6 days after 2 days germination at 22 °C. Seedling growth was observed (upper panel; scale bar, 1 cm) and the hypocotyl length was measured and calculated (bottom panel). Data were presented as means±s.e.m. (n>50) and statistical analysis was performed using student's t-test (**P<0.01, ptre1, tir1–1 and axr1–3 compared with wild type). Mutants axr1–3 and tir1–1 are shown as positive controls. (b) Reduced or enhanced PTRE1 expression altered the expressions of IAA genes (IAA1–6 are shown). Seven-day-old seedlings of wild-type (Col), ptre1 and PTRE1-overexpressing (p35S:PTRE1-GFP) seedlings were analysed and relative expression was calculated using ACTIN2 as a reference gene. Error bars represent s.d. (n=3). The experiments were repeated three times.