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. 2016 Apr 28;6:25069. doi: 10.1038/srep25069

Figure 4. In vitro siRNA delivery efficacy of the screened materials.

Figure 4

(a) Luciferase gene silencing efficacies of the five most efficient materials in HeLa-luc cells. 50 nM siLuc is used for each transfection. (b) Luciferase gene silencing in MDA-MB231-luc cells. 10 nM siLuc is used for each transfection. (c) Bcl-2 gene silencing in HeLa cells. 50 nM siBcl-2 is used for each transfection. (d) PHD-2 gene silencing in NIH3T3 cells. 50 nM siPHD-2 is used for each transfection. (e) Smurf1 gene silencing in hard-to-transfect cells. Primary mouse mesenchymal stem cells were transfected with 50 nM siSmurf1. The gene silencing experiments continue for 24 h. (f) Efficacies of E9-2 in siSmurf1 delivery into primary mouse mesenchymal stem cells. Lipo 2000 is used as a control. Error bars in a-f represent the s.e. (n = 3). *P < 0.05, **P < 0.01, ***P < 0.005 analyzed by student’s t-test.