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. 2016 Apr 28;6:25305. doi: 10.1038/srep25305

Table 1. Summary of pyrosequence reads (Mean ± SE, n = 3).

Samples Effective Reads
No. of OTUs*
Shannon
Chao1
Summer Winter Summer Winter Summer Winter Summer Winter
CK 52168 ± 3277ab 42258 ± 5938a 1753 ± 34c 2305 ± 179a 8.68 ± 0.19c 9.15 ± 0.03a 2473 ± 32b 3033 ± 424a
N50 70601 ± 9344b 46590 ± 1751a 1271 ± 35a 2063 ± 24a 7.22 ± 0.08a 8.76 ± 0.04a 1892 ± 30a 2664 ± 42a
N80 39304 ± 3664a 41373 ± 7851a 1333 ± 78ab 2061 ± 152a 7.52 ± 0.29ab 8.74 ± 0.15a 1941 ± 93a 2577 ± 260a
N100 61562 ± 4887b 50485 ± 457a 1497 ± 37b 1959 ± 129a 7.82 ± 0.04b 8.57 ± 0.32a 2269 ± 84b 2522 ± 157a

*Operational taxonomic units (OTUs) were defined at 97% sequence identity. 100N: normal application rate of N, P2O5, and K2O was 185, 46, and 185 kg ha−1, respectively; 80N: 0.8 times N a as in the 100N; 50N: 0.5 times N as in the 100N; CK: no N applied. Means followed by the same letter within a column are not significantly different as determined by the LSD test (P = 0.05).