Figure 6. 1D Proton NMR Spectra of WT MAGE-A4 and its cancer-associated mutants.

(a) Whole Spectra. (b) Zoomed-in spectra within 0.7 and −2 ppm. Each protein was concentrated to 200 μM in 50 mM phosphate buffer (pH 7.2) containing 50 mM NaCl and 5 mM DTT. The spectra were recorded at 600 MHz, 25 °C.