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. 2016 Apr 28;6:25016. doi: 10.1038/srep25016

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(A) Projected (extended focus) fluorescence images (40 × 60 μm; upper panel) of control monocytes migrating in a loose collagen type I matrix (0.8 mg/ml collagen), obtained by time-lapse spinning disk confocal microscopy, and corresponding reconstruction of 3D morphology (lower panel). (B) Projected (extended focus) fluorescence images (40 × 60 μm; upper panel) of a monocyte migrating in the presence of the ROCK inhibitor Y-27632, and corresponding reconstruction of 3D morphology (lower panel). (C) 3D morphologies of monocytes in the presence of Y-27632 at two time points.