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. 2016 Apr 25;7:11423. doi: 10.1038/ncomms11423

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(a–d) Extension of the G1/swarmer lifetime in a ΔglnD strain can be compensated by addition of glutamine. Motility (a), growth (b) and DNA content (c) of wild-type (WT; RH50) and ΔglnD (RH577) grown in complex media without (PYE) or with glutamine (PYEQ) media. (d) G1/swarmer lifetime is extended in glutamine auxotrophic mutants. Doubling time, motility and G1 proportion were measured in WT (RH50), ΔglnD (RH577), ΔglnB (RH771), ΔglnA (RH772), ΔglnE (RH874), ΔglnD ΔglnE (RH875) and ΔntrC (RH1458) grown in complex media without (PYE) or with glutamine (PYEQ), and normalized to the WT (100%). All these phenotypes can be rescued by addition of glutamine (PYEQ). Error bars=s.d.; n=3.