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. 2016 Apr 25;7:11423. doi: 10.1038/ncomms11423

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Copyright © 2016, Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved.

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(a) Schematic representation of the PTS^Ntr pathway in C. crescentus. (b) The PTS^Ntr components EI^Ntr, HPr and EIIA^Ntr control G1/swarmer lifetime. G1 proportion was measured in wild-type (WT; RH50), ΔspoT (RH1755), ΔptsP (RH1758), ptsP_L83Q (RH1748), ΔptsH (RH1621), ΔptsN (RH1819), ptsN_H66A (RH2019), ptsN_H66E (RH2017), ptsP_L83Q ptsN_H66A (RH2018), ΔptsP ptsN_H66E (RH2016) grown in complex media (PYE) and normalized to the WT (100%). Error bars=s.d.; n=3. (c,d) (p)ppGpp accumulation is modulated by PTS^Ntr. Intracellular levels of (p)ppGpp detected by TLC after nucleotides extraction of WT (RH50), ΔspoT (RH1755), ΔptsP (RH1758), ΔptsH (RH1621), ΔptsN (RH1819) and ptsN_H66A (RH2019) grown (c) in +N or (d) −N conditions. Error bars=s.d.; n=3. Statistically significant differences by Student's t-test in comparison with the WT are indicated as *P<0.05% and **P<0.01% (n=3).