Fig. 5.

Proliferation effects of chSCF isoforms. A) Growth curves of PGCs under several culture conditions. PGCs (1.0 × 10⁴ cells/ml) were seeded onto feeder cells and cultured for 20 days in PGC culture medium containing 5 ng/ml FGF2. Data are presented as the mean ± SEM. Asterisks indicate statistical significance based on the Tukey-Kramer method (*P < 0.05; **P < 0.01). B) PGCs (1.0 × 10⁴ cells/ml) were seeded onto feeder cells and cultured for 8 days using medium with or without FGF2. Data are presented as the mean ± SEM. Asterisks indicate statistical significance based on Welch’s t-test (*P < 0.05; **P < 0.01).