Table 1. Primers used in RT-qPCR.
| Gene | Sequence (5’–3’) | GenBank accession no. | bp | ||
| Glyceraldehyde-3-phosphate dehydrogenase (Gapdh) | Reference | For | TGTGTCCGTCGTGGATCTGA | NM_008084.3 | 150 |
| Rev | TTGCTGTTGAAGTCGCAGGAG | ||||
| Heat shock protein 1A (Hspa1a) | Target | For | GTGGTTGCACTGTAGGACTTGTTTC | NM_010479.2 | 102 |
| Rev | GACCCGAGTTCAGGATGGTTG | ||||
| Myosin, light polypeptide kinase (Mylk) | Target | For | CTGCTGTAGCTCAACCCAGATCC | NM_139300.3 | 109 |
| Rev | ACCGCTTCCAGAATGAGACACC | ||||
| ATPase, Na+/K+ transporting, alpha 1 polypeptide (Atp1a1) | Target | For | CTGATCAGCATGGCCTATGGAC | NM_144900.2 | 87 |
| Rev | ACCGTTCTCAGCCAGAATCACA |
For, forward; Rev, reverse.