Fig. 2.

Deletion analysis of the rat Fshb promoter region (-2824 to +28 b regions) in LβT2 cells with or without treatment with 10 µM unsaturated LCFAs oleic acid, α-linolenic acid, or docosahexaenoic acid (DHA). On the left, reporter constructs are shown containing serial deletion mutants of the Fshb promoter fused with the secreted alkaline phosphatase (SEAP) gene in the pSEAP2-Basic vector that were transfected into LβT2 cells. On the right, SEAP activities are represented as the activity relative to that of the basic vector. Values are the mean ± SEM for four independent experiments. * P < 0.05 vs. pSEAP2-Basic (Dunnett’s method).