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. 2016 Apr 14;8(4):100. doi: 10.3390/v8040100

Figure 7.

Figure 7

NIC02 inhibits primed elongation activity of rabbit calicivirus RdRps in vitro. A gel-based RdRp activity assay was used to evaluate effects of NIC02 on the primed elongation activity of RHDV and RCV RdRps. (a) PE46-C template. (b) Products generated in the in the presence of 19.9 (IC50) and 99.5 µM (5× IC50) NIC02 for RHDV RdRp, 13.5 (IC50) and 67.5 µM (5× IC50) NIC02 for RCV RdRp or in the presence of the vehicle (0.5% v/v DMSO) alone (no inhibitor). Products were separated on a 15% denaturing polyacrylamide gel and stained with SYBR green II. Inhibition of the RdRp of Φ6 bacteriophage was carried out using 200 µM of the nucleoside analogue chain terminator 3′dGTP as an inhibition control.