. 2016 Apr 14;8(4):100. doi: 10.3390/v8040100

Table 1.

Sequences of primer pairs for generation of expression constructs.

Construct Name	Primer Sequence (5′→3′) ^a
RdRp (RHDV)_His₆	F: ATGACGTCAAACTTCTTCTGTGG
RdRp (RHDV)_His₆	R: TATCCTCGAGCTCCATAACATTCACAAATTCGTC
RdRp (RCV)_His₆	F: ATGACTGCAAACTTCTTCTGTG
RdRp (RCV)_His₆	R: TATCCTCGAGCTCCATAACATTCACAAAATCGTC
RdRp (NoV)_His₆	F: TTTAAGAAGGAGATATACATATGGGAGGTGACAGTAAAGGGAC
RdRp (NoV)_His₆	R: CAGTGGTGGTGGTGGTGGTGCTCGAGCTCGACGCCATCTTCATTC

^a RHDV Czech strain V351, RCV-A1 MIC-07 and NoV NSW028D/January/2013 genome sequences (GenBank accession numbers KF594473.1, EU871528.1 and KT239579, respectively) were used to design primers for PCR amplification. F—forward primer; R—reverse primer. NdeI and XhoI restriction sites are underlined. Initiation codon sequences are shown in bold.