Table 2.
Biochemical Property | RHDV RdRp | RCV RdRp | NoV RdRp |
---|---|---|---|
Vmax (pg(dsRNA) × min −1 × ng(RdRp) −1) at 30 °C | 4.0 ± 0.4 | 13.4 ± 1.5 | 4.1 ± 0.6 |
Vmax (pg(dsRNA) × min −1 × ng(RdRp) −1) at 42 °C (RHDV/RCV) or 37 °C (NoV) | 6.6 ± 0.6 | 30.3 ± 1.3 | 5.8 ± 0.3 |
Km (poly(C)) (ng/μL) at 30 °C | 0.5 ± 0.1 | 0.9 ± 0.2 | 3.6 ± 0.6 |
Km (rGTP), Michaelis-Menten model (μM) at 30 °C | 14.6 ± 2.4 | 5.7 ± 0.7 | 77.7 ± 14.5 |
Km (rGTP), substrate inhibition model (μM) at 30 °C | 19.1 ± 3.8 | 8.0 ± 0.8 | 448.8 ± 120.4 |
Ki (rGTP), substrate inhibition model (mM) at 30 °C | 2.1 ± 0.4 | 1.7 ± 0.2 | 0.6 ± 0.2 |