Skip to main content
. 2016 Apr 14;8(4):100. doi: 10.3390/v8040100

Table 2.

Kinetic analysis of the RHDV, RCV and NoV RdRps.

Biochemical Property RHDV RdRp RCV RdRp NoV RdRp
Vmax (pg(dsRNA) × min −1 × ng(RdRp) −1) at 30 °C 4.0 ± 0.4 13.4 ± 1.5 4.1 ± 0.6
Vmax (pg(dsRNA) × min −1 × ng(RdRp) −1) at 42 °C (RHDV/RCV) or 37 °C (NoV) 6.6 ± 0.6 30.3 ± 1.3 5.8 ± 0.3
Km (poly(C)) (ng/μL) at 30 °C 0.5 ± 0.1 0.9 ± 0.2 3.6 ± 0.6
Km (rGTP), Michaelis-Menten model (μM) at 30 °C 14.6 ± 2.4 5.7 ± 0.7 77.7 ± 14.5
Km (rGTP), substrate inhibition model (μM) at 30 °C 19.1 ± 3.8 8.0 ± 0.8 448.8 ± 120.4
Ki (rGTP), substrate inhibition model (mM) at 30 °C 2.1 ± 0.4 1.7 ± 0.2 0.6 ± 0.2