Figure 4.

Impact of ESCRT-III family members on MLV.Gag^YFP release. HuH-7 cells were transfected with siRNAs targeting the indicated ESCRT-III genes and retransfected with MLV.Gag^YFP. In cases where single siRNA duplexes were used, the target gene is designated with a star. The degrees of ESCRT-III knockdowns were measured by qRT-PCR. PCR runs were performed in duplicate, and error bars are standard error of the mean (top panel). Synthesis (Cell) and release (SPN) of MLV.Gag^YFP were probed by GFP-specific WB. Uniformity of sample loading was assessed by anti-ß-Actin WB (middle panel). VLPs values were quantified by densitometric analysis and demonstrated in percent amount relative to control cells in the graph below. Error bars indicate the standard deviations from the mean of two experiments (bottom panel).