Figure 2.

sgG-2 + adjuvant intra-muscularly (i.m.) immunized mice all survived HSV-2 challenge with minimal disease. Mice were immunized three times i.m. with sgG-2 with, and without CpG and alum, with CpG and alum alone, with origin binding protein (OBP) of HSV-1 and CpG and alum, or with phosphate buffered saline (PBS). Mice were also immunized with sgG-2 combined with CpG, once subcutaneously followed by two intranasal administrations. Mice were challenged with 25 × LD₅₀ of HSV-2 333 (10⁵ PFU) intra-vaginally (day 0). (A) The survival rate; and (B) disease score were followed for 21 days post challenge. Data are shown from two separate experiments except for PBS vaccinated control groups which were pooled from three experiments.