Figure 3.

Antibody and CD4+ T-cell responses from sgG-2 + adjuvant i.m. immunized mice. (A) Serum was subjected to an anti-sgG-2 IgG subclass specific enzyme-linked immunosorbent assay (ELISA); (B) Enriched CD4+ T cells from sgG-2 + adjuvant i.m. immunized mice and PBS vaccinated control mice were stimulated with 3 μg/mL sgG-2 for 4 days and proliferation was presented as SI units (international system of units); (C) interferon- γ (IFN-γ) production in the supernatants of stimulated cells was measured with an ELISA. The results are based on two to three separate experiments. Mean values and error bars + standard error of the mean (SEM) are indicated.