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. 2016 Apr 25;8(4):117. doi: 10.3390/v8040117

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© 2016 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

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Mutation of all glutamic acids within p6 inhibits VLP release and Gag maturation. (A) HeLa cells were transiently transfected with pNLenv1 wt or the Glu mutants E6-29A and E0A. VLP release was quantified by western blot time course analyses; (B) The percentage of VLPs released over time was calculated as the amount of CA detected in the VLP fraction versus the total amount of Gag recovered from cell and VLP fractions; (C) Gag processing was calculated as the amount of CA relative to the total amount of Gag in the VLP fraction. Values on the y-axis was adjusted to 1 for wt at t = 0.