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. 2016 Apr 25;8(4):117. doi: 10.3390/v8040117

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© 2016 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

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Mutation of glutamic acids reduces the infectivity and replication capacity of HIV-1. (A) TZM-bl cells were infected with VSV-G pseudotyped pNLenv1 VLP stocks, standardized for p24. Infectious titers were determined by measuring the β-galactosidase activity. Bars represent the mean values of 3 independent experiments ± SD; (B) Infection of human lymphoid aggregate cultures (HLAC) with 1 ng virus standardized for p24 of HIV-1_NL4-3 wt or the respective Glu mutants. Replication was assessed by quantification of the reverse transcriptase activity in cell culture supernatant collected every second or third day post infection (dpi) during 15 days of cultivation.