Figure 5.

Defect in virus release of the E0A mutant cannot be rescued by ALIX overexpression. (A) HeLa cells were co-transfected with pNLenv1 expression plasmids coding for wt, ∆PTAP or E0A and with FLAG-ALIX or empty control plasmids, respectively. VLP fractions and whole cell lysates were analyzed by western blot using an anti-CA. ALIX expression was detected using anti-FLAG antibodies; (B) VLP release was calculated as the amount of CA in the VLP fraction relative to the total amount of Gag detected in cells and VLPs. Bars represent the mean values of 3 independent experiments ± SD. Values on the y-axis were adjusted to 100% for wt.