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. 2016 Mar 25;8(4):90. doi: 10.3390/toxins8040090

Figure 2.

Figure 2

Interaction of the TcsL-cat mutants with BPS as monitored by ELISA. (A) TcsL-cat mutants with a drastic decrease in binding to BPS as compared to the deletion of the 18 N-terminal residues (TcsL 19–543); (B) TcsL-cat mutants with a moderate decrease in binding to BPS; (C) TcsL-cat mutants without significant change in binding to BPS; (D) FAR UV spectra of TcsL-cat mutants and TcsL wild-type. Spectra of TcsL-cat mutants with a drastic (Figure 2A) and moderate (Figure 2B) decrease in binding to BPS are shown. Proteins were diluted in Hepes 20 mM, NaCl 150 mM, TCEP 1 mM, pH 7.4.