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. 2016 Mar 25;8(4):90. doi: 10.3390/toxins8040090

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© 2016 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

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Glucosylation activity of TcsL-cat wt towards Ras in solution versus cellular membrane extracts enriched in Ras. Recombinant Ras (7 ng) or membrane-bound Ras (7 ng as assayed by ELISA) were used in each glucosylation test with serial dilutions of TcsL-cat in the presence of UDP-[₁₄C]glucose. The reactions were performed at 37 °C for 30 min. One representative experiment from 3 is shown.