Figure 5.

cI mRNA transcript levels in MG1655 lysogens. Quantitative real-time PCR was used to determine P_RM cDNA levels produced from reverse transcription of total RNA extracted MG1655 lysogenized with 933W (white bars), BAA2326 (black bars), λ (stippled bars), or 434 (hatched bars). Amounts of P_RM transcripts were normalized to the amount of uid transcripts determined in parallel reverse transcription reactions (see Methods and Materials). The amount of P_RM transcripts produced by Stx⁺ prophages (933W and BAA2326) are significantly lower (* p ≤ 0.01) than those produced by Stx⁻ (λ and 434) prophages.