Table 1.
Description of de novo assembly and analysis of the C. fuscescens tentacle transcriptome.
| Assembly | Count |
|---|---|
| Raw reads (paired-end) | 26,991,925 |
| After cleaning | 17,319,746 |
| Contigs | 30,317 |
| Average length ± SD | 628.70 ± 840.07 |
| Length (min and max) | 201 to 31,945 |
| GC content | 40.42% |
| Raw reads mapped to contigs | 97.69% |
| CDS | Count |
| Containing a coding region | 23,534 (78%) |
| Transcripts with significant BLAST hit (1 × 10−5) | 16,925 (72%) |
| With homologues in databases: | |
| GenBank non-redundant Cnidarian protein sequences | 15,987 (53%) |
| H. vulgaris | 14,261 (47%) |
| SwissProt | 13,375 (44%) |
| N. vectensis | 12,144 (40%) |
| Uniprot animal toxin and venom | 549 (2%) |
| Sequence analysis | Count |
| Returning GO term | 11,586 (49%) |
| GO terms returned: | |
| Molecular function | 8265 (35%) |
| Biological process | 4768 (20%) |
| Cellular component | 2173 (9%) |
| Predicted proteins with signal sequences * | 1012 (4%) |
| Predicted proteins with two or more transmembrane helices | 641 (2%) |
* SignalP on top hit from SwissProt returned 1666 (7%).