Table 2.
Putative toxins and venom-related proteins identified in the C. fuscescens venom proteome using MS/MS.
| Transcript | Category | Unique Peptides (n) | Coverage (%) | InterProScan Protein Feature | Signal Peptide (Yes/No) | Transmembrane Domain (Yes/No) | BLAST Analysis | |
|---|---|---|---|---|---|---|---|---|
| Protein Identity | Species of Closest Homology | |||||||
| comp13691_c0_seq2 | Metalloprotease | 18 | 76.6 | Peptidase M1, alanine aminopeptidase | n | n | Aminopeptidase N-like | H. vulgaris |
| comp12218_c0_seq1 | Metalloprotease | 16 | 76.2 | Peptidase M13 | n | y (27–49) | Endothelin-converting enzyme 1-like | H. vulgaris |
| comp13767_c0_seq1 | Metalloprotease | 10 | 80.4 | Peptidase M13 | y (1–29) | y (12–29) | Endothelin-converting enzyme 2-like | H. vulgaris |
| comp11996_c0_seq1 | Metalloprotease | 8 | 62.2 | Peptidase M1, alanine aminopeptidase | n | n | Endoplasmic reticulum aminopeptidase 1-like | H. vulgaris |
| comp11571_c0_seq2 | Metalloprotease | 8 | 75.4 | Peptidase M13 | n | n | Endothelin-converting enzyme 1-like | H. vulgaris |
| comp10942_c1_seq1 | Metalloprotease | 7 | 71.0 | Peptidase M13 | n | y (31–53) | Endothelin-converting enzyme 1-like | H. vulgaris |
| comp12208_c0_seq1 | Metalloprotease | 6 | 61.0 | Peptidase M14, carboxypeptidase A | y (1–20) | y (292–309) | Carboxypeptidase D-like | H. vulgaris |
| comp9530_c0_seq2 | Metalloprotease | 6 | 87.3 | Peptidase M13 | n | n | Endothelin-converting enzyme 1-like | N. vectensis |
| comp14393_c0_seq1 | Metalloprotease | 5 | 68.2 | Peptidase M2, peptidyl-dipeptidase A | n | n | Angiotensin-converting enzyme-like isoform | H. vulgaris |
| comp14137_c0_seq1 | Metalloprotease | 2 | 42.1 | Peptidase M2, peptidyl-dipeptidase A | n | n | Angiotensin-converting enzyme | H. vulgaris |
| comp14070_c0_seq1 | Metalloprotease | 3 | 67.3 | Peptidase M18 | n | y (493–510) | Aspartyl aminopeptidase-like | Lepisosteus oculatus |
| comp13494_c0_seq1 | Aspartyl protease | 2 | 39.6 | Aspartic peptidase | y (1–17) | y (161–193) | Cathepsin D | Pteria penguin |
| comp12883_c0_seq1 | Aspartyl protease | 2 | 48.9 | Aspartic peptidase | y (1–18) | y (2–24) | Aspartyl protease | Placozoa sp. H4 |
| comp13655_c0_seq2 | Serine protease | 2 | 67.8 | Peptidase S8/S53 | n | y (156–178, 323–339, 748–771) | PC3-like endoprotease variant B isoform X1 | H. vulgaris |
| comp13207_c0_seq1 | Pore-forming toxin | 6 | 75.4 | Delta-endotoxin, N-terminal | y (1–19) | y (104–125) | Toxin TX2 | A. aurita |
| comp12925_c0_seq1 | Pore-forming toxin | 8 | 76.0 | Delta endotoxin, N-terminal | n | y (15–37) | Uncharacterized protein LOC105843890 | H. vulgaris |
| comp13855_c0_seq5 | Venom allergen | 37 | 83.0 | Cysteine-rich secretory protein, allergen V5/Tpx-1-related | n | n | Cell wall protein PRY3-like | H. vulgaris |
| comp13672_c0_seq1 | Venom allergen | 18 | 81.4 | Cysteine-rich secretory protein, allergen V5/Tpx-1-related | n | n | Cell wall protein PRY3-like | H. vulgaris |
| comp13791_c0_seq1 | Venom allergen | 15 | 75.2 | Cysteine-rich secretory protein, allergen V5/Tpx-1-related | n | n | Cell wall protein PRY3-like | H. vulgaris |
| comp13791_c0_seq3 | Venom allergen | 9 | 70.6 | Cysteine-rich secretory protein, allergen V5/Tpx-1-related | n | n | Cell wall protein PRY3-like | H. vulgaris |
| comp13342_c1_seq2 | Venom allergen | 2 | 76.1 | Cysteine-rich secretory protein, allergen V5/Tpx-1-related | n | n | Cell wall protein PRY3-like | H. vulgaris |
| comp12264_c0_seq1 | Venom allergen | 2 | 81.7 | Cysteine-rich secretory protein, allergen V5/Tpx-1-related | y (1–20) | y (7–29) | PRY2-like protein | Pyronema omphalodes CBS 100304 |
| comp13629_c0_seq1 | C-type lectin | 194 | 85.0 | C-type lectin | y (1–20) | y (344–362) | Golgi-associated plant pathogenesis-related protein 1 | H. vulgaris |
| comp13792_c0_seq2 | C-type lectin | 44 | 70.0 | C-type lectin | n | y (96–116) | Golgi-associated plant pathogenesis-related protein 1 | H. vulgaris |
| comp13880_c0_seq1 | C-type lectin | 8 | 56.5 | C-type lectin | y (1–21) | y (348–368) | Golgi-associated plant pathogenesis-related protein 1 | H. vulgaris |
| comp13219_c0_seq1 | Glycoside hydrolase | 18 | 59.4 | Beta-hexosaminidase | y (1–20) | y (416–438) | beta-hexosaminidase subunit alpha-like isoform X1 | H. vulgaris |
| comp7130_c0_seq1 | Enzyme inhibitor | 4 | 64.0 | Peptidase S8/S53 domain | y (1–19) | y (484–507) | Tripeptidyl-peptidase 1-like | H. vulgaris |