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. 2016 Apr 13;8(4):109. doi: 10.3390/toxins8040109

Figure 1.

Figure 1

Divalent metal ion-activated glycosyltransferase activity of C. sordellii lethal toxin (TcsL). Rac1 (50 µg/mL) and rN-TcsL (0.3 nM) or rN-TcdB (0.3 nM) as indicated were incubated in the presence of the indicated divalent metal ions (1 mM) at 37 °C for 10 min. [14C]glucosylated Rac1 was separated by SDS-page and visualized by autoradiography.