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. 2016 Apr 13;8(4):109. doi: 10.3390/toxins8040109

Figure 2.

Figure 2

Divalent metal ion-activated UDP-glucose hydrolysis activity of C. sordellii lethal toxin (TcsL). (A) UDP-[14C]glucose (100 µM) was incubated in the presence of 0.1 M·HCl with Mn2+ (1 mM) and rN-TcsL (100 nM) as indicated at 37 °C for 30 min. UDP-[14C]glucose and [14C]glucose were separated by thin layer chromatography on PEI cellulose and visualized by autoradiography. (B) UDP-[14C]glucose (100 µM) was incubated in the presence of rN-TcsL (100 nM) with the indicated divalent metal ions (1 mM) at 37 °C for 30 min. Signal intensities of formed [14C]glucose were quantified and are given as means ± SD (n = 3).