Table 1.
Neu-Reagent UV/365 nm | ||||
---|---|---|---|---|
Band N° | Rf | Color | Type of Phenol/Possible Flavonoid | |
Gq | 1 | 0.03 | Blue-white | Flavonols, flavones, isoflavones, flavonones |
2 | 0.05 | Orange | Flavonols glycosides | |
3 | 0.09 | Yellow-green | Favonols | |
4 | 0.14 | Yellow-pale | Flavonols, flavones, isoflavones, flavonones | |
5 | 0.18 | Blue | Phenolic acid | |
6 | 0.26 | Blue | Flavonoïd aglycones | |
7 | 0.32 | Orange | Flavonols, flavones, isoflavones, flavonones | |
8 | 0.36 | Red | Methylated flavones | |
9 | 0.42 | Orange | Not identified | |
10 | 0.50 | Yellow | Phenolic acid | |
11 | 0.55 | Yellow-pale | Phenolic acid | |
12 | 0.78 | Blue fluorescent | Phenolic acid | |
Tp | 1′ | 0.05 | Blue-white fluorescent | Phenolic acid |
2′ | 0.12 | Yellow-green | Not identified | |
3′ | 0.19 | Blue-white | Phenolic acid, isoflavones, flavonones | |
4′ | 0.26 | Yellow-orange | Flavonols | |
5′ | 0.31 | Yellow-green | Flavonols, flavones, isoflavones, flavonones | |
6′ | 0.36 | Orange | Flavonols-glycosides | |
7′ | 0.44 | Red | Not identified | |
8′ | 0.54 | Blue-white | Not identified | |
9′ | 0.98 | Blue-white fluorescent | Phenolic acid |
TLC-fingerprint analysis conditions: Eluent: ethyl acetate/ethanol/formic acid/water (100:11:11:26); Support: Merck TLC silica gel 60 F254 and Detection: under UV light in 365 nm after revelation with Neu-reagent (1%). Rf: Retention factor.