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. 2016 Apr 12;8(4):215. doi: 10.3390/nu8040215

Figure 7.

Figure 7

Autophagy is modulated by glutamine deficiency. (A) pMACs were treated with BSA-PBS or palm-LPS in glutamine sufficient or glutamine deficient media ± α-KG (440 nM). The cells were incubated for 16 h followed by 2 h of veh or bafilomycin (BAF; 50 nM). Protein lysates were analyzed for expression of LC3 or p62. Actin is shown as a loading control; (B) Cells were stimulated as indicated for 16 h in media with or without glutamine followed by qRT-PCR assessment of LC3 and p62 mRNA expression. Bar graphs report the mean ± SE for a minimum of 3 experiments, each performed in triplicate. *, p < 0.05 for PBS vs. LPS; #, p < 0.05 glutamine vs. no glutamine.