Table 1.
Primer sequences for real time quantitative PCR
| Primer | Sequencea | Coding region | Product size | Annealing temperature |
|---|---|---|---|---|
| GAPDH | 5′‐GAG GGT AGT GAA GGC TGC TG‐3′ (forward) | 504–523 | 200 bpb | 55°C |
| 5′‐CCA CAA CAC GGT TGC TGT AT‐3′ (reverse) | 684–703 | 55°C | ||
| MyoD | 5′‐GAC GGC ATG ATG GAG TAC AG‐3′ (forward) | 553–572 | 201 bp | 58°C |
| 5′‐AGC TTC AGC TGG AGG CAG TA‐3′ (reverse) | 734–753 | 58°C | ||
| MyoG | 5′‐CCT TTC CCA CTC CTC TCC AAA‐3′ (forward) | 813–833 | 175 bp | 58°C |
| 5′‐GAC CTT GGT CGA AGA GCA ACT‐3′ (reverse) | 967–987 | 58°C | ||
| MRF4 | 5′‐AGG CTC TGA AAA GGA GGA CTG T ‐3′ (forward) | 341–362 | 307 bp | 58°C |
| 5′‐AGG CTG CTG GAA GCC GAC GAC T ‐3′ (reverse) | 626–647 | 58°C |
a
Primer sequences were designed from the following GenBank accession numbers: glyceraldehyde‐3‐phosphate dehydrogenase (GAPDH), U94327.1; myogenic differentiation 1 (MyoD), AY641567.1; Myogenin (MyoG), AY560111.3; Myogenic regulatory factor 4 (MRF4) GU223068.
b
Base pairs of DNA.