. Author manuscript; available in PMC: 2017 Mar 1.

Published in final edited form as: Curr Protoc Mouse Biol. 2016 Mar 1;6(1):39–66. doi: 10.1002/9780470942390.mo150178

Table 1. Primers Used in the studies.

Oligonucleotides used in a PCR reaction to generate the DNA templates for in vitro transcription of the Cas9 mRNA and sgRNA

Gene	Direction	Sequence (5′ to 3′)	Reference
Cas9	F	TAATACGACTCACTATAGGGAGACCACCATGGACTATAAGGACCACGAC	(Cong et al., 2013)
Cas9	R	GCGAGCTCTAGGAATTCTTAC	(Cong et al., 2013)
sgRNA, conventional backbone	F1	gaaattaatacgactcactatag(N20)gttttagagctagaaatagc	(Cong et al., 2013)
sgRNA, conventional backbone	R1	aaaagcaccgactcggtgccactttttcaagttgataacggactagccttattttaacttgctatttctagctctaaaac	(Cong et al., 2013)
sgRNA improved backbone	F2	taatacgactcactatag(N20)gtttaagagctatgctggaaac	(Chen et al., 2013)
sgRNA improved backbone	R2	aaaaaagcaccgactcggtgccactttttcaagttgataacggactagccttatttaaacttgctatgctgtttccagcatagctcttaaac	(Chen et al., 2013)