Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
| Component | Stock (ng/μL) | Volume (μL) | Final (ng/μL) | Comments |
|---|---|---|---|---|
| Cas9 mRNA | 500 | 3 | 100 | |
| sgRNA | 500 | 1.5 | 50 | |
| Oligonucleotide | 500 | (3) | 100 | Only needed for HDR model |
| Plasmid | 500 | (0.6) | 20 | Only needed for HDR model |
| TE | variable | Added to achieve the 15 μL total volume | ||
| Total | 15 |
